Since the appearance of ragweed pollen (Ambrosia sp.) is a general health problem, often associated with the spread of invasive species A. artemisiifolia L. noticed in the city of Dubrovnik, the aim of this study was to determine whether and to what extent ragweed pollen is present in the air in Dubrovnik and if so, to explore its pollination seasons features. The study was performed in the period from 2005 to 2009 using the standard volumetric method of airborne pollen sampling by Hirst-type sampler. Ragweed pollen was present in the atmosphere of Dubrovnik with an average annual share less than 1%. However, in 2006 that share was 1% and in 2008 was 1.2%. Ragweed pollen was present in the air during the summer and fall. The longest pollen season was recorded in 2007 (56 days) and the highest pollen concentration in 2009 (48 pg/m3). The results of the Spearman's correlation test between meteorological parameters and ragweed pollen counts show a significant correlation, positive with temperature (mean, maximum and minimum) and negative with rainfall and wind speed. Positive effect was noted between the relative air humidity and the ragweed pollen count, except in 2006, when this effect was negative.