In recent years, with the rapid development of medical research, cancer diagnosis and treatment technology have significantly improved young cancer patient's survival rate. Anticancer therapy such as chemotherapy, radiotherapy, or hematopoietic stem cell transplantation can lead to premature ovarian insufficiency. The endocrine and reproductive function of the ovary is critical to women's physical and mental health. Ovarian tissue cryopreservation and transplantation can protect not only female fertility but also preserve ovarian endocrine function. This paper interprets the guidelines for ovarian tissue cryopreservation and transplantation issued by the Chinese Society of Gynecological Endocrinology affiliated to the International Society of Gynecological Endocrinology. The purpose of this guideline's interpretation is to promote more medical workers to understand the technology of ovarian tissue cryopreservation and transplantation, which can provide patients with more choices of fertility protection methods and improve their quality of life.