Simultaneous Determination of Azorubin and New Red by Synchronous Fluorescence Spectra Coupled with Radial Basis Function Neural Networks.
- Resource Type
- Article
- Authors
- Kong, Fanbiao; Chen, Guoqing; Zhu, Chun; Li, Run; Hu, Yangjun; Zhang, Yong; Zhu, Zhuowei
- Source
- Spectroscopy Letters. 2015, Vol. 48 Issue 4, p296-301. 6p.
- Subject
- *FLUORESCENCE spectroscopy
*ARTIFICIAL neural networks
*MONOCHROMATORS
*EMISSION spectroscopy
*STANDARD deviations
- Language
- ISSN
- 0038-7010
A method for the simultaneous determination of Azorubin and New Red was proposed, which used the synchronous fluorescence spectroscopy coupled with the radial basis function neural networks. The synchronous fluorescence spectra of 59 standards of Azorubin and New Red mixed with concentrations ranging from 5 to 20 µg mL−1were obtained by synchronous scanning the excitation and emission monochromator maintained at an offset of 70 nm. A method of exploiting the complicated function relationship between the concentrations of Azorubin and New Red and the intensities of their mixtures was discussed according to the accuracies of the radial basis function neural networks predicting results. The final root mean square error is 0.1922 and the total mean relative error is 2.56%. Results show that the method developed is effective, simple, and of satisfactory accuracy. [ABSTRACT FROM AUTHOR]