Environmental regulation of plant gene expression: An RT-qPCR laboratory project for an upper-level undergraduate biochemistry or molecular biology course.
- Resource Type
- Article
- Authors
- Eickelberg, Garrett J.; Fisher, Alison J.
- Source
- Biochemistry & Molecular Biology Education; Sep/Oct2013, Vol. 41 Issue 5, p325-333, 9p
- Subject
- Biochemistry education in universities & colleges
Molecular biology study & teaching
Science education (Higher)
Data analysis
Gene expression in plants
Plant molecular genetics
Arabidopsis thaliana
Gene expression
- Language
- ISSN
- 14708175
We present a novel laboratory project employing 'real-time' RT-qPCR to measure the effect of environment on the expression of the FLOWERING LOCUS C gene, a key regulator of floral timing in Arabidopsis thaliana plants. The project requires four 3-hr laboratory sessions and is aimed at upper-level undergraduate students in biochemistry or molecular biology courses. The project provides students with hands-on experience with RT-qPCR, the current 'gold standard' for gene expression analysis, including detailed data analysis using the common [ABSTRACT FROM AUTHOR]