AIMS: To identify further Western blot bands that may be specific in the diagnosis of Lyme borreliosis. METHODS: The Borrelia burgdorferi antibody profiles of 270 western blot positive patients and 241 western blot negative patients from 2008 were examined. RESULTS: 27 different non-specific bands were detected in both groups. Six of 27 (22%) of the non-specific bands were detected significantly more in the western blot positive patients compared to the western blot negative patients (20Â kDa, p<0.0001; 28Â kDa, p<0.002; 36Â kDa, p<0.002; 37Â kDa, p<0.007; 48Â kDa, p<0.023; 56Â kDa, p<0.028; two-tailed F test). CONCLUSION: Results suggest that the 20, 28 and 48Â kDa bands should be regarded as specific. [ABSTRACT FROM AUTHOR]