The article presents a discussion on the conserved residues in the Pseudomonas aeruginosa type IV pilus motor protein PilB, PilT, and PilU's characteristic secretion NTPase motifs as well as their functional role. It is noted that type IV pili can be considered as retractable protein fibers for adherence, host colonization, biofilm development, and twitching motility which is used by several bacterial pathogens. In Pseudomonas aeruginosa, the PilB and PilT are bipolar proteins that belong to the superfamily of secretion NTPase.