Purpose: This study aimed to find a standard of the vertebra kinematics during functional weight-bearing activities in degenerative lumbar scoliosis (DLS) patients. Methods: Fifty-four patients were involved into this study with forty-two in DLS group and twelve in the control group. The three-dimensional (3D) vertebral models from L1 to S1 of each participant were reconstructed by computed tomography (CT). Dual-orthogonal fluoroscopic imaging, along with FluoMotion and Rhinoceros software, was used to record segmental vertebral kinematics during functional weight-bearing activities. The primary and coupled motions of each vertebra were analyzed in patients with DLS. Results: During flexion–extension of the trunk, anteroposterior (AP) translation and craniocaudal (CC) translation at L5–S1 were higher than those at L2–3 (9.3 ± 5.1 mm vs. 6.4 ± 3.5 mm; P < 0.05). The coupled mediolateral (ML) translation at L5–S1 in patients with DLS was approximately three times greater than that in the control group. During left–right bending of the trunk, the coupled ML rotation at L5–S1 was higher in patients with DLS than that in the control group (17.7 ± 10.3° vs. 8.4 ± 4.4°; P < 0.05). The AP and CC translations at L5–S1 were higher than those at L1–2, L2–3, and L3–4. During left–right torsion of the trunk, the AP translation at L5–S1 was higher as compared to other levels. Conclusions: The greatest coupled translation was observed at L5–S1 in patients with DLS. Coupled AP and ML translations at L5–S1 were higher than those in healthy participants. These data improved the understanding of DLS motion characteristics. [ABSTRACT FROM AUTHOR]