Prokaryotic expression of the V protein of the peste des petits ruminants virus and development of an indirect ELISA.
- Resource Type
- Article
- Authors
- Lu, Guili; Wang, Ping; Miao, Shukui; Huang, Jiong; Ma, Wenge; Mi, Xiaoyun; Xue, Jing; Shayilan, Kayizha; Yang, Xueyun; Yan, Genqiang
- Source
- Animal Biotechnology. Dec2023, Vol. 34 Issue 9, p5011-5015. 5p.
- Subject
- *PESTE des petits ruminants
*PROTEIN expression
*RECOMBINANT proteins
*NEUTRALIZATION tests
- Language
- ISSN
- 1049-5398
In this study, we recombinantly expressed the V protein of the peste des petits ruminants virus (PPRV) and evaluated its diagnostic value for PPRV infection using an indirect ELISA (i-ELISA). The optimal concentration of the coated antigen of V protein was 15 ng/well at a serum dilution of 1:400, and the optimal positive threshold value was 0.233. A cross-reactivity assay showed that the V protein-based i-ELISA was specific to PPRV with consistent reproducibility and showed a specificity of 82.6% and a sensitivity of 100% with a virus neutralization test. Using the recombinant V protein as an antigen in ELISA is useful for seroepidemiological studies of PPRV infections. [ABSTRACT FROM AUTHOR]