Dual-Labeled Octahedral Gold and Magnetic Microsphere Electrochemical Immunosensor for Ultrasensitive Determination of Carbohydrate Antigen-199 (CA199) by Differential Pulse Voltammetry (DPV).
- Resource Type
- Article
- Authors
- Wang, Hongdan; Yuan, Wei; Zhang, Liqiu; Xie, Baogang; Cheng, Qiong; Zhang, Ningwen
- Source
- Analytical Letters. 2024, Vol. 57 Issue 5, p841-854. 14p.
- Subject
- Language
- ISSN
- 0003-2719
To precisely detect CA199 antigen, a novel electrochemical immunosensor based on horseradish peroxidase (HRP) and glucose oxidase (GOD) double enzyme system was prepared. Thiol was adsorbed on the surface of nano-octahedral gold through Au-S self-assembly; the second antibody was labeled by glutaraldehyde ligase as a signal amplification reagent. Amino magnetic microspheres were used to connect GOD and bovine serum albumin (BSA) through glutaraldehyde. The biosensor displayed low detection limits of 0.2 U/mL and wide linear range (0.5–200 U/mL). This novel immunosensor improves the sensitivity of current detection, showing promising application prospects in the clinical immunoassay detection of CA199. [ABSTRACT FROM AUTHOR]