Sample pretreatment is essential for trace analysis of pesticides in complex food and environment matrices. Recently, organic–inorganic hybrid materials have gained increasing attention in pesticide extraction and preconcentration. This review highlighted the common organic–inorganic hybrid materials used as absorbents in sample pretreatment for pesticide detection. Furthermore, the preparation and characterization of organic–inorganic hybrid materials were summarized. To obtain a deep understanding of adsorption toward target analytes, the adsorption mechanism and absorption evaluation were discussed. Finally, the applications of organic–inorganic hybrid materials in sample pretreatment techniques and perspectives in the future are also discussed. [ABSTRACT FROM AUTHOR]