3D printing creates physical objects from a geometrical representation by successive addition of materials in a layer-by-layer fashion directly from a computer-aided design (CAD) model. It is a fast-emerging technology widely used all over the globe. Fused Deposition Technique/Modelling (FDM) is one of the 3D printing technologies for rapid prototyping which uses Polylactic Acid (PLA), Acrylonitrile Butadiene Styrene (ABS), etc., where it produces a tissue scaffold by melt extrusion of thermoplastic polymer. In this work, the impact resistance measurements studied on the 3D printed Polylactic Acid (PLA) material under varied layer thicknesses and extrusion temperatures are presented. It is observed that an optimized layer thickness is carefully be considered to induce optimum properties to the component over its build times. [ABSTRACT FROM AUTHOR]