Characterization of Ca2+-ATPase, LMCA1, with native cell membrane nanoparticles system.
- Resource Type
- Article
- Authors
- Dhankhar, Poonam; Trinh, Thi Kim Hoang; Qiu, Weihua; Guo, Youzhong
- Source
- BBA: Biomembranes. Jun2023, Vol. 1865 Issue 5, pN.PAG-N.PAG. 1p.
- Subject
- *CELL membranes
*CALCIUM ions
*MEMBRANE proteins
*LISTERIA monocytogenes
*NANOPARTICLES
*ADENOSINE triphosphatase
- Language
- ISSN
- 0005-2736
Ca2+-ATPases are membrane pumps that transport calcium ions across the cell membrane and are dependent on ATP. The mechanism of Listeria monocytogenes Ca2+-ATPase (LMCA1) in its native environment remains incompletely understood. LMCA1 has been investigated biochemically and biophysically with detergents in the past. This study characterizes LMCA1 using the detergent-free Native Cell Membrane Nanoparticles (NCMNP) system. As demonstrated by ATPase activity assays, the NCMNP7-25 polymer is compatible with a broad pH range and Ca2+ ions. This result suggests that NCMNP7-25 may have a wider array of applications in membrane protein research. [ABSTRACT FROM AUTHOR]