The experiment was performed to determine the antioxidant enzymes viz. superoxide dismutase (SOD), catalase (CAT), peroxidase (POx), and glutathione S-transferase (GST) activities in different organs viz. muscle (M), heart (H), brain (B), kidney (K), liver (L), and gills (G) of Cirrhina mrigala, Catla catla and Labeo rohita exposed to 1/3rd of LC50 of endosulfan (E), chlorpyrifos(C) and bifenthrine(B) mixture for 60 days. Geno-toxicity in term of DNA damage, micronuclei and other nuclear abnormalities in erythrocytes of each fish were also observed. Results showed that the CAT activity of C. catla and L. rohita was increased in L, G, K and M during first 15 days after that CAT level decreased upto 60-day. However, same was decreased in B and H of exposed fish species as compared to control. Similarly, C. mrigala had increased CAT activity in G, L, K and B during first 15-day after that it was decreased. However, CAT activity decreased in H and M of exposed fish in relation to control. The GST, POx and SOD activities were accelerated significantly in all organs of exposed three fish species during first 30-days after that GST activity decreased significantly. The trend of GST activity in organs of fish species was studied as L>B>K>G>H>M. C. catla showed higher GST and SOD activity than L. rohita and C. mrigala. Species specific response of POx showed the order as: L. rohita>C. catla>C. mrigala. POx activity was maximum in L followed by B, G, K, H and M. Organs specific response of SOD followed the order: L>B>K>G>H>M. The results of genotoxicity indicated that DNA damage, MN and NAs in erythrocytes of each fish increased during first 30 days of exposure after that time dependent decreased. In conclusion, antioxidant enzymes and genotoxic parameters are the useful tools to determine the toxic effect of aquatic xenobiotics. [ABSTRACT FROM AUTHOR]