Improving cross-protection against influenza virus in mice using a nanoparticle vaccine of mini-HA.
- Resource Type
- Article
- Authors
- Zhu, Hechao; Li, Xiangmin; Ren, Xujiao; Chen, Huanchun; Qian, Ping
- Source
- Vaccine. Oct2022, Vol. 40 Issue 44, p6352-6361. 10p.
- Subject
- *INFLUENZA A virus
*INFLUENZA viruses
*TRANSMISSION electron microscopy
*ANTIBODY titer
*NANOPARTICLE size
*IMMUNOGLOBULINS
- Language
- ISSN
- 0264-410X
• The mini-HA-LS nanoparticles were assembled optimally via SpyTag/SpyCatcher when the mass ratio of SC-LS and the soluble mini-HA proteins was 1:4. • The mini-HA-LS nanoparticles units could be seen as small spheres with a diameter of about 60–80 nm by TEM. DLS analyses indicated that they have a relatively regular assembly rather than disorderly aggregation. • The mini-HA-LS nanoparticles had good immunogenicity, which could enhance antibody titers and cellular immune responses effectively. • The IgG antibodies which could recognize influenza virions and SIV-infected cells were induced by the mini-HA-LS nanoparticles. • Mice immunized with mini-HA-LS nanoparticles could be protected against cross-clade influenza virus challenges. This study aimed to investigate the protective effect of mini-hemagglutinin (mini-HA) proteins expressed on lumazine synthase (LS) nanoparticles against influenza. Soluble mini-HA proteins were assembled with LS proteins via SpyTag/SpyCatcher in vitro. The size of mini-HA-LS nanoparticles was characterized by transmission electron microscopy (TEM) and dynamic light scattering (DLS), and the effect of mini-HA-LS nano-vaccines was explored in mice. The results indicate that the diameter of mini-HA-LS nanoparticles was approximately 60–80 nm. The nanoparticles could induce stronger humoral and cellular immune responses and produce cross-clade protection against influenza in mice. [ABSTRACT FROM AUTHOR]