The temperature sensitivity (Q 10) of soil respiration (Rs) is crucial to assess the carbon (C) budget of terrestrial ecosystems under global warming. The Q 10 changes along a climatic gradient as well as its seasonal dynamics remain unclear, and the underlying microbial mechanisms are not well known. Here, the seasonal Q 10 of Rs at the northern, middle, and southern sites of a natural temperate mixed forest was examined. The mean annual temperature (MAT) of the sampling sites spanned from 0.5 to 4.9 °C. The Q 10 pattern over the climatic zones was highly dependent on season, with Q 10 increasing toward the southern region in spring and autumn, but having a similar level across the sampling sites in summer. In spring, Q 10 was independent of microbial community composition and functions. Instead, spring Q 10 increased with decreasing C availability from north to south, consistent with the Carbon-Quality-Temperature theory. In summer, Q 10 was closely associated with the dominance of microbial r -strategy features, characterized by high copiotroph/oligotroph and labile/recalcitrant C degradation gene ratios. In autumn, however, Q 10 was driven by the K -selected microbial communities, which might have been ascribed to the priming effects mediated by fresh plant litter. The seasonality of Q 10 was site-dependent. The southern and middle sites had the lowest Q 10 in summer, consistent with the Seasonal Plasticity Hypothesis , which predicts lower temperature sensitivity in warmer seasons. In contrast, the Q 10 at the northern site remained stable during the growing season due to minor seasonal fluctuations in plant litter inputs and microbial community composition and functions. This work deepens our understanding on the complex relationships between Q 10 , carbon availability and microorganisms over spatial and temporal scales by translating microbial phylogenetic data into life strategies. • Spatial change in Q 10 varied by season and seasonality of Q 10 are site dependent. • Q 10 increased towards south site in spring and autumn but remain similar in summer. • Spring Q 10 decreased with C availability and showed weak linkages with microbes. • Summer and Autumn Q 10 was associated with microbial r- and K-strategy, respectively. • South region had low Q 10 in summer and north region had seasonally stable Q 10. [ABSTRACT FROM AUTHOR]