Introduction: Platelet plays an important role during wound healing as it is rich in growth factors such as platelet-derived growth factor. It was observed that Cord Blood-Platelet Lysate (CB-PL), harvested from human umbilical cord blood, has similar efficacy to Peripheral Blood-Platelet Lysate (PB-PL) in initiating the cell growth and differentiation which makes it a unique alternative to implementing in oral ulceration healing. This research study compares the effectiveness of cord blood platelet lysate gel (CB-PLG) and peripheral blood-platelet lysate gel (PB-PLG) in promoting the healing of oral ulcers. Methods: Normal oral mucosal fibroblasts were taken during minor oral surgery procedures and underwent a cell culture process. Cord blood was collected through the in utero method, while the peripheral blood was collected from the healthy donors. The blood underwent double centrifugation to obtain the platelet-rich plasma and was analysed for platelet count. The platelet was subjected to three freeze and thaw cycles to produce PL. In order to determine the optimal concentration of CB-PLG and PB-PLG, Alamar Blue Assay was used to examine the cell proliferation of human oral mucosal fibroblasts. Subsequently, a real-time polymerase chain reaction determined the vimentin gene expression in the fibroblasts of CB-PLG and PB-PLG groups. Conclusion: It is postulated that CB-PL is as effective as PB-PL and can be used as an alternative to promote the healing of oral ulcers. [ABSTRACT FROM AUTHOR]