Hydrophilic metal-organic frameworks integrated uricase for wearable detection of sweat uric acid.
- Resource Type
- Article
- Authors
- Xiao, Jingyu; Luo, Yong; Su, Lei; Lu, Jiafa; Han, Wei; Xu, Tailin; Zhang, Xueji
- Source
- Analytica Chimica Acta. May2022, Vol. 1208, pN.PAG-N.PAG. 1p.
- Subject
- *METAL-organic frameworks
*PERSPIRATION
*ELECTROCHEMICAL sensors
*ELECTRONIC equipment
*DETECTION limit
*BIOMACROMOLECULES
- Language
- ISSN
- 0003-2670
The chemistry of the metal-organic frameworks (MOFs) coating may affect the biological functionality of the encapsulated biomacromolecules in harsh environment. Enzymes encapsulated in hydrophilic MAF-7 can retain high activity in harsh environment. We conducted this study to prepare a non-invasive wearable uircase@MAF-7-based electrochemical sensor that can achieve accurate and sensitive detection of UA levels in sweat by integrating a flexible microfluidic chip and wireless electronic readout device. The flexible microfluidic chip enabled an easy and effective collection of sweat samples. MAF-7 protected enzyme activity by encapsulating uricase. The uricase@MAF-7-based electrochemical sensor enabled the highly sensitive detection of UA in the concentration range of 2 μM–70 μM with a detection limit of as low as 0.34 μM. Additionally, we evaluated the utility of the sensor for monitoring UA levels in real sweat samples by means of a high purine dietary challenge. This personalized wearable sweat sensing device has a potential to be used for monitoring disease-related metabolites in daily life. [Display omitted] • The flexible microfluidic chip enabled an easy and effective collection of sweat. • We embedded uricase into hydrophilic MAF-7 using the one-pot strategy. • This wearable sweat sensing device can monitor disease-related metabolites. [ABSTRACT FROM AUTHOR]