Antimicrobial spray for material coating was developed using polymer particles containing quaternary ammonium (QA) and benzophenone (BP) groups on their surfaces. The obtained particles can be coated onto substrate with a covalent bond via the BP group, and the QA group displays antimicrobial behavior. First, the macro chain transfer agent (macro CTA) of poly(2-methacryloyloxy dodecyl dimethyl ammonium chloride-4-allyloxy-2-hydroxybenzophenone)-iodide (P(QAC12-BP)-I) was synthesized by solution iodine transfer polymerization. It was then used as a macro CTA for the preparation of poly(styrene-butyl methacrylate) [P(S-BA)] particles by emulsion iodine transfer polymerization. The obtained P(S-BA) particles were nearly uniform (0.090 of polydispersity index; PDI), having a size of about 101 nm. A positive charge of +70 mV was observed due to the presence of the QA group derived from P(QAC12-BP) segments on their surfaces. In addition, the QA group on the particle surface represented antimicrobial activity against microorganisms such as Staphylococcus aureus and Escherichia coli. This particle can coat fabrics via UV initiation without a binder and demonstrates high washing durability, with over 80% of particles remaining after 10 washing cycles. [ABSTRACT FROM AUTHOR]