Lipids have diverse functions in regulating the plasma membrane's cellular processes and signaling mediation. Plasma membrane lipids are also involved in the plant's complex interactions with the surrounding microorganisms, with which plants are in various forms of symbiosis. The roles of lipids influence the whole microbial colonization process, thus shaping the rhizomicrobiome. As chemical signals, lipids facilitate the stages of rhizospheric interactions – from plant root to microbe, microbe to microbe, and microbe to plant root – and modulate the plant's defense responses upon perception or contact with either beneficial or phytopathogenic microorganisms. Although studies have come a long way, further investigation is needed to discover more lipid species and elucidate novel lipid functions and profiles under various stages of plant root–microbe interactions. Lipids have diverse functions across the different stages in the formation of plant root–microbe interactions, commencing from the shaping of the rhizomicrobiome to the establishment of symbiosis. Plant rhizodeposition and microbe-generated signals involve lipid substances, which act as chemical signals that are exchanged for successful microbe recruitment or phytopathogen defense. The plasma membrane, as the interface for plant roots and microbes, is where lipids' intrinsic functions modulate and facilitate interactions. [ABSTRACT FROM AUTHOR]