Keywords: tobacco; NIC1; ERF; gene editing; low nicotine EN tobacco NIC1 ERF gene editing low nicotine 2150 2152 3 10/26/21 20211101 NES 211101 Nicotine is the predominant alkaloid in tobacco plants, accounting for ~90% of their total alkaloid content. Furthermore, the ultra-low-nicotine levels in Mut-T1 confirmed that I ERF199 i is the only causal gene for nicotine biosynthesis within the I NIC1 i locus. NIC1 cloning and gene editing generates low-nicotine tobacco plants Tobacco, NIC1, ERF, gene editing, low nicotine. [Extracted from the article]