Two new species of the genus Glenea Newman, 1842 are described from south India. Glenea vellayaniensis sp. nov. is described from Vellayani, a suburb of Trivandrum district, along the coastline of Kerala, and G. pseudoalbosignatipennis sp. nov. is described from the southern Western Ghats. Glenea vestalis Heller, 1934, previously treated as a junior synonym of G. pulchella Pascoe, 1858, is reinstated as a valid species owing to its differences in morphological and genital characters; in addition, both species are illustrated and revised. Glenea vellayaniensis sp. nov. is provisionally placed under Glenea sensu lato and compared with its morphologically similiar congeners, G. pulchella Pascoe, 1858 and G. vestalis Heller, 1934 along with comments on its generic affinity. Glenea pseudoalbosignatipennis sp. nov. is compared with G. albosignatipennis Breuning, 1950 and G. signaticollis Gahan, 1889 and a note on its life history is provided. [ABSTRACT FROM AUTHOR]