Objective. By attaching absorbent patches to the skin to collect sweat, an increase in local skin temperature (Tsk) underneath the patches seems unavoidable. Yet this effect has not been quantified. The present study investigates the effect of absorbent patch application on local Tsk underneath. Approach. Ten healthy participants cycled for 60 min at an exercise intensity relative to their body surface area (40 W.m−2) in three environmental conditions (temperate: 25 °C 45% RH, hot-humid: 33 °C 65% RH and hot-dry: 40 °C 30% RH). The effect of short sweat sampling (i.e. from min 25–30 to min 55–60) on Tsk was examined on the right scapula. Tsk of the left scapula served as control. The effect of continuous sweat sampling (i.e. four consecutive 15 min periods) on Tsk was examined on the right upper arm. Tsk of the left upper arm served as control. Main results. Neither short nor continuous application of absorbent sweat patches affected Tsk underneath the patches in the hot-humid and hot-dry condition (P > 0.05). In the temperate condition, continuous application led to a significant increase in Tsk underneath the patches during the first and second minute. This increase remained throughout the experiment (1.8 ± 0.6 °C; P < 0.001). Short application of sweat patches did not affect the local Tsk underneath (P > 0.05) in the temperate condition. Significance. To avoid a significant increase in local Tsk underneath sweat patches, continuous application should be prevented in, especially, a temperate condition. Timely removal of sweat patches should be taken into account during longer periods of collecting sweat in field or laboratories settings. [ABSTRACT FROM AUTHOR]