Surface modification with linker molecules is necessary for the effective immobilization of bioreceptors and for improving the performance of biosensors. In this work, we present a novel functionalization technique to attach amine (NH 2) linker on graphene-based sensor surfaces. This is achieved by reacting the commercially available reduced graphene oxide (rGO) screen-printed electrodes (SPEs) with ammonia solution at room temperature. The NH 2 linkers are attached predominantly on the edge and defect sites of rGO SPE through chemisorption as shown by XPS, Raman and FTIR analysis. The functionalized SPEs are further characterized using morphological and electrochemical techniques. The validation of the linker is done by using the functionalized SPEs for the detection of Aβ 1-40 and Aβ 1-42 biomarkers. A limit of detection (LOD) of 9.51 fM is achieved over a linear dynamic range of 10 fM-10 pM for Aβ 1-40. Similarly, LOD of 8.65 fM is achieved over a linear dynamic range of 10 fM-50 pM for Aβ 1-42. This excellent sensitivity is attributed to the functionalization of rGO surface with NH 2 linker, which provides a large number of binding sites for bioreceptors. High specificity for the target biomarkers over interfering Aβ and ApoE ε4 species is also demonstrated. The biosensor is further validated for the analysis of spiked human plasma. The proposed technique provides a promising approach for improving the detection sensitivity of graphene biosensors for application in biofluids based minimally invasive and cost- and time-efficient disease diagnosis. Figure: Fabrication process of ultrasensitive amine (NH 2) functionalized reduced graphene oxide (rGO) biosensor: (a) rGO SPE; (b) modification with amines; (c) incubation in antibody and protein G mixture; (d) blocking with BSA and (e) attachment of antigen. [Display omitted] • First report on functionalization of reduced graphene oxide electrode with NH 2 linker via chemisorption at room temperature. • Demonstration of femtomolar detection of Aβ 1-40 and Aβ 1-42 with a label-free biosensor functionalized with NH 2 linker. • Lowest reported limit of detection with excellent linear dynamic range and specificity for label-free biosensors. • Confirmation of applicability of biosensor for biofluidic analysis using spiked human plasma. [ABSTRACT FROM AUTHOR]