Characterization of Anoectochilus roxburghii polysaccharide and its therapeutic effect on type 2 diabetic mice.
- Resource Type
- Article
- Authors
- Gao, Huashan; Ding, Linlin; Liu, Rui; Zheng, Xinhua; Xia, Xichao; Wang, Fuan; Qi, Jinxu; Tong, Weishuang; Qiu, Yuanhao
- Source
- International Journal of Biological Macromolecules. May2021, Vol. 179, p259-269. 11p.
- Subject
- *TREATMENT effectiveness
*TYPE 2 diabetes
*WESTERN immunoblotting
*POLYSACCHARIDES
*HIGH-fat diet
*INSULIN resistance
- Language
- ISSN
- 0141-8130
Anoectochilus roxburghii is a traditional herb in China that can be potentially used to treat diabetes. A novel polysaccharide ARLP-W was isolated from Anoectochilus roxburghii by chromatography on DEAE-52 cellulose. Chemical analysis indicated that ARLP-W (8.1 × 104 Da) was mainly composed of mannose and glucose. The main linkages of glycosidic bonds of ARLP-W were β-1, 4-Manp and α-1, 4-Glcp. The terminal Glcp was connected to Manp-via O-3. RT-qPCR and western blotting analysis showed that ARLP-W caused a significant reduction in the levels of the key gluconeogenesis enzymes phosphoenolpyruvate carboxykinase (PEPCK) and glucose 6-phosphatase (G6Pase) in the liver. The results of the insulin resistance tests indicated that ARLP-W increased glucose absorption. These results indicate that ARLP-W has a good therapeutic effect on type 2 diabetes and can assist with further development and application treatment of diabetes. • A new homogeneous polysaccharide ARLP-W was isolated from Anoectochilus roxburghii. • The structures of polysaccharide ARLP-W was investigated. • ARLP-W improves insulin resistance and serum indexes on high-fat diet and STZ-induced T2DM model. • ARLP-W inhibits the expression of key gluconeogenesis enzymes PEPCK and G6Pase. [ABSTRACT FROM AUTHOR]