Genome editing, tomato, CRISPR, Cas9, prime editing The prime editing system consists of three parts: an nCas9-MMLV (engineered Moloney murine leukaemia virus reverse transcriptase) fusion protein, a prime editing guide RNA (pegRNA) and a small guide RNA (sgRNA) for nicking. Keywords: prime editing; genome editing; tomato; CRISPR; Cas9 EN prime editing genome editing tomato CRISPR Cas9 415 417 3 03/15/21 20210301 NES 210301 The CRISPR/Cas-mediated genome editing technology has been widely applied to create knockout alleles of genes by generating short insertions or deletions (indel) in various plant species. [Extracted from the article]