Withanolides from Physalis peruviana showing nitric oxide inhibitory effects and affinities with iNOS.
- Resource Type
- Article
- Authors
- Dong, Bangjian; An, Lijun; Yang, Xueyuan; Zhang, Xuke; Zhang, Jie; Tuerhong, Muhetaer; Jin, Da-Qing; Ohizumi, Yasushi; Lee, Dongho; Xu, Jing; Guo, Yuanqiang
- Source
- Bioorganic Chemistry. Jun2019, Vol. 87, p585-593. 9p.
- Subject
- *CAPE gooseberry
*NITRIC-oxide synthases
*TIME-dependent density functional theory
*WITHANOLIDES
*NITRIC oxide
*MOLECULAR docking
- Language
- ISSN
- 0045-2068
• Five new withanolides were isolated from Physalis peruviana. • The structures were elucidated by NMR data and ECD calculations. • Compounds 2 – 4 showed promising NO inhibitory effects. • Molecular docking revealed strong affinities of bioactive compounds with iNOS. A phytochemical study to obtain new nitric oxide (NO) inhibitors resulted in the isolation of five new withanolides from the whole plants of Physalis peruviana. The structures were determined on the basis of extensive NMR spectroscopic data analysis as well as the time-dependent density functional theory (TDDFT) electronic circular dichroism (ECD) calculations. The NO inhibitory effects were examined by inhibiting NO release in lipopolysaccharide-stimulated murine microglial BV-2 cells. Molecular docking studies showed the strong interactions of bioactive compounds with the inducible nitric oxide synthase (iNOS) protein, revealing the potential mechanism of NO inhibition of bioactive compounds. [ABSTRACT FROM AUTHOR]