In situ fluorescent profiling of living cell membrane proteins at a single-molecule level.
- Resource Type
- Article
- Authors
- Fan, Yuanyuan; Li, Lu; Lu, Meng; Si, Haibin; Tang, Bo
- Source
- Chemical Communications. 4/11/2019, Vol. 55 Issue 28, p4043-4046. 4p.
- Subject
- *MEMBRANE proteins
*CELL membranes
*PROTEIN analysis
- Language
- ISSN
- 1359-7345
We propose a signal amplification method that enables visualization analysis of membrane proteins on living cells at a single-molecule level. Using the proposed method, we achieved imaging of PTK7 membrane proteins on HeLa cells and analyzed the down-regulated expression of EpCAM on the MCF-7 cell surface during epithelial–mesenchymal transition (EMT). [ABSTRACT FROM AUTHOR]