Localization Microscopy of Actin Cytoskeleton in Human Platelets.
- Resource Type
- Article
- Authors
- Mayr, Sandra; Hauser, Fabian; Peterbauer, Anja; Tauscher, Andreas; Naderer, Christoph; Axmann, Markus; Plochberger, Birgit; Jacak, Jaroslaw
- Source
- International Journal of Molecular Sciences. Apr2018, Vol. 19 Issue 4, p1150. 10p. 1 Color Photograph, 1 Black and White Photograph, 1 Graph.
- Subject
- *CYTOSKELETON
*PHALLOIDINE
*ACTIN
*RHODAMINES
*FLUOROPHORES
- Language
- ISSN
- 1661-6596
Here, we measure the actin cytoskeleton arrangement of different morphological states of human platelets using a new protocol for photo-switching of rhodamine class fluorophores. A new medium composition was established for imaging the cytoskeleton using Alexa Fluor 488 conjugated to phalloidin. Morphological states of platelets bound to a glass substrate are visualized and quantified by two-dimensional localization microscopy at nanoscopic resolution. Marker-less drift correction yields localization of individual Alexa 488 conjugated to phalloidin with a positional accuracy of 12 nm. [ABSTRACT FROM AUTHOR]