N6-(2-Hydroxyethyl)-adenosine (HEA), which was the first calcium antagonist derived from biological sources, has been intensively investigated because of its ability to inhibit tumour cell proliferation, restrain inflammation, protect kidneys and function as a sedative and insecticide. Up to now, the production of HEA has been detected only in a few species such asCordyceps pruinosa, C. militarisandIsaria tenuipes. Here, we found a new HEA-producing fungus, which was identified asBeauveria bassianabased on morphological and phylogenetic characteristics. The HEA production was verified by reversed-phase high-performance liquid chromatography/mass spectrometry in this fungus. Moreover, HEA was also detected in the mycelia of two otherB. bassianastrains from different origins, but not in the culture medium of all testedB. bassianastrains. The maximum production of HEA (0.8483 ± 0.0439 mg/g mycelia dry weight) was achieved on day 7. The fungal biomass and kinetics of HEA production exhibited similar trends over the duration of the culture period. This is the first report describing HEA production inB. bassiana, which suggests that this fungal strain may have new applications as a source of HEA. [ABSTRACT FROM AUTHOR]