Antihypertensive effects of Ile-Pro-Ile-Lys from krill ( Euphausia superba) protein hydrolysates: purification, identification and in vivo evaluation in spontaneously hypertensive rats.
- Resource Type
- Article
- Authors
- Park, Soo; Je, Jae-Young; Kang, Nalae; Han, Eui; Um, Ju; Jeon, You-Jin; Ahn, Ginnae; Ahn, Chang-Bum
- Source
- European Food Research & Technology. Apr2017, Vol. 243 Issue 4, p719-725. 7p.
- Subject
- *EUPHAUSIA superba
*ANTIHYPERTENSIVE agents
*PROTEIN hydrolysates
*IN vivo studies
*LABORATORY rats
- Language
- ISSN
- 1438-2377
Angiotensin I-converting enzyme (ACE) inhibitory peptide was purified from krill ( Euphausia superba) protein hydrolysate using consecutive chromatography using an SP-Sephadex C-25 cation exchanger and then reverse-phase high-performance liquid chromatography. The purified ACE inhibitory peptide was identified to be Ile-Pro-Ile-Lys with a molecular weight of 470.3 Da by LC-MS/MS. The IC value of Ile-Pro-Ile-Lys was 57.4 µM. Lineweaver-Burk plots suggest that Ile-Pro-Ile-Lys acts as a non-competitive inhibitor against ACE. Oral administration of Ile-Pro-Ile-Lys significantly ( p < 0.05) decreased the systolic blood pressure of spontaneously hypertensive rats. Collectively, an antihypertensive peptide purified from krill protein hydrolysates may be useful as a food ingredient for treatment of hypertension and related diseases. [ABSTRACT FROM AUTHOR]