Lecanicillium saksenaeCGMCC5329 is a useful biological control agent against plant-parasitic nematodes. The complete mitogenome sequence ofL. saksenaeis reported for the first time. The mitochondrial genome is 25 919 bp long with 14 typical protein-coding genes, an intronic ORF coding for a putative ribosomal protein (rps3), 2 ribosomal RNA genes and a set of 26 transfer RNA genes. The phylogeny based on 12 protein-coding genes (except the loss of other two genes inAcremonium implicatum) suggests the close phylogenetic relationship betweenL. saksenaeandL. muscarium. Comparative analysis reveals that mitogenome ofL. saksenaeis 1420 bp larger thanL. muscarium, mainly due to the intergenic region betweencox2andtrnR. ThetrnCbetweencobandcox1is conserved in the mitogenomes of three nematophagous fungus ofPochonia chlamydosporia, A. implicatumandL. saksenae, but absent inL. muscarium. This study may provide valuable information for further research on mitochondrial evolution of nematophagous fungus andLecanicilliumspecies. [ABSTRACT FROM AUTHOR]