Enzymatic conversion of magnetic nanoparticles to a non-magnetic precipitate: a new approach to magnetic sensing.
- Resource Type
- Article
- Authors
- Kolhatkar, Arati G.; Jamison, Andrew C.; Nekrashevich, Ivan; Kourentzi, Katerina; Litvinov, Dmitri; Brazdeikis, Audrius; Willson, Richard C.; Randall Lee, T.
- Source
- Analyst. 9/21/2016, Vol. 141 Issue 18, p5246-5251. 6p.
- Subject
- *MAGNETIC nanoparticles
*BIOMOLECULES
*ALKALINE phosphatase
*PHOSPHATASES
*NANOPARTICLES
- Language
- ISSN
- 0003-2654
Magnetic sensing utilizes the detection of biomolecule-conjugated magnetic nanoparticles (MNPs). Our new strategy offers a novel approach to magnetic sensing where in situ conversion produces a “loss of signal” in the sensing device. This report demonstrates the enzymatic conversion of Fe3O4 MNPs to a non-magnetic precipitate via reduction by l-ascorbic acid generated by the action of alkaline phosphatase. [ABSTRACT FROM AUTHOR]