Substrate specificity of Mortierella alpina Δ9-III fatty acid desaturase and its value for the production of omega-9 MUFA.
- Resource Type
- Article
- Authors
- Hao, Guangfei; ChEN, Haiqin; Yang, Bo; Du, Kai; Wang, Hongchao; Gu, ZhENnan; Zhang, Hao; ChEN, Wei; ChEN, Yong Q.
- Source
- European Journal of Lipid Science & Technology. May2016, Vol. 118 Issue 5, p753-760. 8p.
- Subject
- *MORTIERELLA
*FATTY acid desaturase
*MONOUNSATURATED fatty acids
*BIOCHEMICAL substrates
*PALMITIC acid
- Language
- ISSN
- 1438-7697
The fatty acid desaturase (FADS9-III) gene from M. alpina ATCC 32222 was cloned and overexpressed in a homologous system. Lipid analysis via GC-MS revealed that the Δ9-III fatty acid desaturase had moderate activity with palmitic acid (C16:0) and stearic acid (C18:0) as substrates. Palmitoleic acid (C16:1) and oleic acid (C18:1) contents increased 4.0- and 1.94-fold in the FADS9-III overexpression clones compared to the wild-type strain. Low temperature (12°C) significantly improved the conversion ratio of C16:0 to C16:1 (9.12-fold increase). In addition, the FADS9-III fatty acid desaturase had specific activity at the ω9 position with lignoceric acid (C24:0) as a substrate. Practical applications: Our results indicate that FADS9-III prefers stearic acid (C18:0) and palmitic acid (C16:0) as substrates and has ω9 fatty acid desaturase activity on lignoceric acid (C24:0). The conversion ratio of C24:0 to C24:1 reached 17% by supplementation of C24:0 in the medium at low temperature. The M. alpina ω9 fatty acid desaturase (FADS9-III) is valuable for the production of mono-unsaturated fatty acids with a double bond at the ω9 position. The M. alpina Δ9-III fatty acid desaturase is valuable for production of MUFA with the double bond at the ω9 position. [ABSTRACT FROM AUTHOR]