m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation.
- Resource Type
- Article
- Authors
- Geula, Shay; Moshitch-Moshkovitz, Sharon; Dominissini, Dan; Mansour, Abed AlFatah; Kol, Nitzan; Salmon-Divon, Mali; Hershkovitz, Vera; Peer, Eyal; Mor, Nofar; Manor, Yair S.; Ben-Haim, Moshe Shay; Eyal, Eran; Yunger, Sharon; Pinto, Yishay; Jaitin, Diego Adhemar; Viukov, Sergey; Rais, Yoach; Krupalnik, Vladislav; Chomsky, Elad; Zerbib, Mirie
- Source
- Science. 2/27/2015, Vol. 347 Issue 6225, p1002-1006. 5p.
- Subject
- *PLURIPOTENT stem cells
*TRANSFERASES
*CELL differentiation
*GREEN fluorescent protein
*SMALL interfering RNA
*MESSENGER RNA
*MOLECULAR switches
*ALLELES
- Language
- ISSN
- 0036-8075
The article focuses on the role of the N6methyladenosine (m6A) transferase Mettl3 in regulating the state transitions of naïve and primed pluripotency toward cell differentiation. It states a small interfering RNA screen was conducted against transcriptional regulators and tested if a primed EpiSC harboring a green fluorescent protein reporter knockin allele might rely on some of the factors. It comments that M6A messenger RNA methylation was a regulator acting at molecular switches.