Immobilization and stabilization of subtilisin Carlsberg in magnetically-separable mesoporous silica for transesterification in an organic solvent.
- Resource Type
- Article
- Authors
- Hwang, Ee Taek; Lee, Byoungsoo; Zhang, Meiling; Jun, Seung-Hyun; Shim, Jongmin; Lee, Jinwoo; Kim, Jungbae; Gu, Man Bock
- Source
- Green Chemistry. 2012, Vol. 14 Issue 7, p1884-1887. 4p.
- Subject
- *MESOPOROUS silica
*TRANSESTERIFICATION
*ORGANIC solvents
*ETHYL esters
*CHEMICAL amplification
- Language
- ISSN
- 1463-9262
Subtilisin Carlsberg (SC) was immobilized and stabilized on magnetically-separable mesoporous silica (Mag-MSU-F) in the form of nanoscale enzyme reactors (NERs) based on the ship-in-a-bottle mechanism. Stabilized NERs of SC (NER-SC) were freeze-dried and successfully used for the transesterification of N-acetyl-l-phenylalanine ethyl ester with n-propanol in isooctane. Magnetic separation of Mag-MSU-F facilitated the repeated usages of stable NER-SC. This is the first demonstration for the use of stable and magnetically-separable NERs in an organic solvent, which has the potential for environmentally-friendly synthesis using enzymes in organic solvents. [ABSTRACT FROM AUTHOR]