The observation of bone targeted anti-osteoporosis effect of free rhubarb anthraquinone using fluorescent traceing method.
- Resource Type
- Article
- Authors
- DENG Yifeng; CHENG Yan; XU Bilian; LIU Yuyu; LIAO Jinming
- Source
- Chinese Journal of Osteoporosis. Oct2014, Vol. 20 Issue 10, p1159-1163. 5p.
- Subject
- Language
- Chinese
- ISSN
- 1006-7108
Objective To investigate the bone targeted anti-osteoporosis effect of free rhubarb anthraquinone compounds in rats with glucocorticoid-induccd osteoporosis. Methods Using fluorescent tracing method, the fluorescence in the epiphysis of the distal femurs was observed in rats with glucocorticoid-induced osteoporosis in situ. It was correlated with bone mineral density (BMD) and bone mineral content (BMC) at the same location. One hundred and twenty-six 4-month-old female SD rats were randomly divided into prednisone group (Pred), prednisone + free anthraquinoncs group (Pred + FAQ), and normal control group (CON). Each group was further subdivided into 7 subgroups. The administration period was 13 weeks at the last. BMD and BMC in distal part (2 mm) of the femurs were scanned using pQCT. The photofluorogram of the coronary symmetrical section of the bone samples in Pred + FAQ group was obtained using cpitluorcsccncc microscope. Results BMD of the distal part of the femur was significantly increased in Pred + FAQ group (P < 0.05) compared to those in Pred group. The fluorescence of the epiphysis of bone samples at all time points elevated or eliminated following the epiphyseal "growth-closed" cycle in Pred +FAQ group. It was not significantly correlated with BMD of the bone samples. BMC was not significantly correlated with BMD of the bone samples. Conclusion Free rhubarb anthraquinonc compounds can prevent bone loss due to glucocorticoidHiiduccd osteoporosis. There is no significant accumulation of fluorescence combined free anthraquinonc compounds and bone targeting property. This experiment explores a beneficial research model for investigate bone targeted anti-osteoporosis effective components in bone tissue through pharmacodynamics/pharmacokinetics link perspective. [ABSTRACT FROM AUTHOR]