Aim: To evaluate the safety and efficacy of an ovine extracellular matrix (ECM) for acute and chronic wounds. Method: We considered 10 patients: 7 affected by chronic ulcers of the leg; 1 affected by second degree burn of the arm, 1 diabetic patient affected by a loss of substance of the fifth left finger and 1 affected by an umbilical loss of substance after postbariatric surgery. After mechanical debridement, we applied the ovine extracellular matrix wet by saline and we covered it with an antiadherent dressing as secondary dressing. We checked the patients after one week and, if necessary, we performed the same kind of dressing every week for a month. Results / Discussion: After the first week all the patients demonstrated an improved healing of the wounds; we obtained an average 90% rehepitelization after 1 month in all the patient (range 75%-100%). No significative differences were noticed between the different etiopathogenesis of the lesions. No adverse events have been reported. Conclusion: In our experience the ovine extracellular matrix has been a safe and effective matrix. We didn't notice any adverse events and no significative differences have been reported between the etiopathogenesis of the lesions. The dressing procedure is easy and unpainful for the patient.