Objective: To evaluate the effect of opportunistic salpingectomy on ovarian reserve and vascularity.Methods: In this observational study, 45 premenopausal women undergoing hysterectomy were divided into two groups: group 1 (22 patients) with salpingectomy and group 2 (23 patients) without salpingectomy. Serum anti-Mullerian hormone (AMH) estimation and transvaginal sonography were done to assess ovarian Doppler indices prior to and 3 months after surgery.Results: Before surgery, median values of AMH (0.45 ng/ml vs 0.44 ng/ml) and ovarian Doppler indices (RI, PI and S/D ratio) were matched in both the groups. The serum AMH levels were decreased by 0.01 ng/ml in group 1 and increased by 0.08 ng/ml in group 2 at 3 months post-surgery (p value 0.19). Positive changes in AMH were seen in 45% versus 65% patients and negative changes in 55% versus 35% patients in both the groups (p value 0.34). Changes (Δ) in RI (− 0.08 vs − 0.15 p value 0.17), PI (− 0.15 vs − 0.17 p value 0.78) and S/D ratio (− 0.39 vs − 0.35 p value 0.84) from baseline to 3 months postoperatively showed a trend towards increased vascularity and were not found to be significantly different between the two groups.Conclusion: Opportunistic salpingectomy did not affect ovarian reserve and vascularity at post 3 months of surgery.