Aim: Access to clean sanitation is closely linked to women’s dignity, health, and wellbeing, and lack of these is a significant concern. We explore the perceptions about the poor sanitation access for on-duty female police officials working at off-site locations and its implications with regard to their behavior, health, and social lives.Subject and methods: We conducted a cross-sectional questionnaire-based survey study with 150 on-duty female police officials in the period January–March, 2020 in South India to elicit their perceptions about access to sanitation in off-site locations, their behavioral modifications to tackle this issue, and its impacts on their health and social lives. Data analysis was done using SPSS software.Results: A high percentage of female police officials (69%) reported not having toilet access while on duty in off-site locations, and 80% avoided drinking water during duty to avoid accessing toilets. Poor toilet access was significantly associated with higher risk of genitourinary symptoms (OR: 2.1; 95% CI: 0.96–4.83) and urinary tract Infections (OR: 2.5; 95% CI: 1.05–5.48). About 11% of participants abstained from work for hygiene purposes during their menstruation owing to discomfort without toilet access, with consequent wage loss and significant adverse social impacts (OR: 10.8; 95% CI: 4.7–25.2; p value = 0.0001).Conclusion: Reduced access to clean sanitation facilities for on-duty female police officials has adverse implications on their toileting and hydration behavior, health, and social lives. Gender-sensitive policies and enhanced welfare facilities are needed to motivate and to obviate health and social risks for the female police force engaged in protecting the country’s citizens.