Nontargeted UHPLC–MS for the Study of the Diversity of Flavonoid Glycosides in Different Fermented Teas
- Resource Type
- Original Paper
- Authors
- Li, Juan; Wang, Haiting; Ma, Tengzhou; Yang, Wuye; Chen, Jieming; Yu, Jing; Wu, Ting; Du, Yiping
- Source
- Chromatographia: An International Journal for Separation Science. 84(6):571-579
- Subject
- Nontargeted
Flavonoid glycosides
Differential analysis
Fermented teas
UHPLC-MS
- Language
- English
- ISSN
- 0009-5893
1612-1112
Although black tea and dark tea are both fermented teas, their fermentation processes are varied a lot and strongly affect their chemical components. The flavonoids which are the major active compounds in teas play an important role in their refreshing effect and health benefit. The differential flavonoid glycosides in these two fermented teas were investigated using nontargeted modification-specific metabolomics UHPLC–MS method. Principal component analysis (PCA) and orthogonal projection on latent structure-discriminant analysis (OPLS–DA) showed that these two types of fermented teas could be clearly distinguished according to their characteristic secondary metabolites. 218 glycosylated records were found out through six specific glycosylated neutral loss calculations. After intersection calculations between glycosylated metabolites and differential secondary metabolites, seven flavonoid glycosides markers were identified which contribute to the classification of two kinds of fermented teas. According to the experimental results, the contents of flavonoid glycosides may strongly relate to the fermented processing technology and microbial fermentation of dark tea would decrease the contents of flavonoid glycosides. The proposed strategy could provide a feasible and reliable technique to understand the glycosylation level under different treatments.