The mass energy absorption coefficient (μen/ρZPEAeffNPEAeffμen/ρZPEAeffNPEAeffZPEAeffZPIeffZPEAeffZPIeff), effective atomic number (μen/ρZPEAeffNPEAeffμen/ρZPEAeffNPEAeffZPEAeffZPIeffZPEAeffZPIeff), and electron density (μen/ρZPEAeffNPEAeffμen/ρZPEAeffNPEAeffZPEAeffZPIeffZPEAeffZPIeff) of some biomolecules with potential application in radiation dosimetry were calculated for their photon energy absorption (PEA) in the energy region of 1–20 MeV. It was noticed that the values of μen/ρZPEAeffNPEAeffμen/ρZPEAeffNPEAeffZPEAeffZPIeffZPEAeffZPIeff, μen/ρZPEAeffNPEAeffμen/ρZPEAeffNPEAeffZPEAeffZPIeffZPEAeffZPIeff, and μen/ρZPEAeffNPEAeffμen/ρZPEAeffNPEAeffZPEAeffZPIeffZPEAeffZPIeff vary with the energy and composition of the biomolecules. The results for μen/ρZPEAeffNPEAeffμen/ρZPEAeffNPEAeffZPEAeffZPIeffZPEAeffZPIeff were compared with effective atomic numbers (μen/ρZPEAeffNPEAeffμen/ρZPEAeffNPEAeffZPEAeffZPIeffZPEAeffZPIeff) owing to the photon interaction (PI). Significant differences were noted between μen/ρZPEAeffNPEAeffμen/ρZPEAeffNPEAeffZPEAeffZPIeffZPEAeffZPIeff and μen/ρZPEAeffNPEAeffμen/ρZPEAeffNPEAeffZPEAeffZPIeffZPEAeffZPIeff in the energy region of 10–150 keV for all of the biomolecules involved. A maximum difference of 45.36% was observed at 50 keV for creatinine hydrochloride. Moreover, the studied attenuation parameters were found to be sharply affected at the K-absorption edge of relatively high-Z elements present in the biomolecules.