In vitro clonal propagation of dioecious Carica papaya
- Resource Type
- Original Paper
- Authors
- Reuveni, O.; Shlesinger, D. R.; Lavi, U.
- Source
- Plant Cell, Tissue and Organ Culture: An International Journal on Biotechnology of Higher Plants. January 1990 20(1):41-46
- Subject
- papaya
endophytic contamination
rifampicin
micropropagation
tissue culture
- Language
- English
- ISSN
- 0167-6857
1573-5044
A procedure for in vitro propagation of dioecious papaya clones is described. A high rate of success in culture estbalishment was obtained when axillary buds were taken from lateral shoots of hedged rooted cuttings grown in a greenhouse. Seasonal endophytic contamination was suppressed by shaking propagules for 24 h in 300 mgl-1 rifampicin or by incorporating it at 50 mgl-1 into the medium. Murashige & Skoog (MS) basal medium supplemented with 0.5 mgl-1 6-benzyladenine and 0.1 mgl-1 naphthaleneacetic acid was used for establishment and proliferation. The addition of 160 mgl-1 adenine sulfate improved multiplication and shoot growth. An elongation stage on MS medium supplemented with 1.0 mgl-1 kinetin and 0.05 mgl-1 naphthaleneacetic acid was necessary before rooting. Rooting was obtained at a high rate on half-strength macroelements of MS medium supplemented with 1.0 mgl-1 indole-3-butyric acid. Commercial plots of papaya plants obtained through this procedure already exist.