Purpose: Aberrant expression of the nuclear division cycle 80 (NDC80) complex has been implicated in various cancers but remains understudied in ovarian cancer (OC). This study aimed to evaluate the mRNA expression of NDC80 complex genes (NDC80, NUF2, SPC24, and SPC25) in OC and explore possible associations with the clinicopathological features of OC patients.Methods: NDC80, NUF2, SPC24, and SPC25 mRNA expression levels were evaluated in 40 OC tissue specimens and 40 adjacent non-cancerous ovarian tissue specimens by real-time quantitative PCR. Correlations of the genes’ expression with the patients’ clinicopathological data were reviewed. Effect of the genes’ expression on survival outcomes was assessed using Kaplan–Meier plotter. Logistic regression analysis was used to identify predictors of higher tumor stages among OC patients.Results: All four genes were significantly overexpressed in OC tissues compared to adjacent non-cancerous ovarian tissues. NDC80, SPC24, and SPC25 demonstrated significant diagnostic values. Combinations of NDC80 + SPC24, NUF2 + SPC24, and SPC24 + SPC25 showed a diagnostic advantage over single-gene analysis. NDC80 and NUF2 were associated with late tumor stages. NUF2 was associated with bilateral ovarian masses. SPC24 was related to negative PR status. The mRNA expression of NDC80, NUF2, and SPC25 was positively correlated. NUF2 overexpression and mutated p53 were significant predictors of higher tumor stages. NDC80, NUF2, and SPC25 predicted poor overall survival. NDC80 and NUF2 predicted poor progression-free survival.Conclusion: Overexpression of NDC80 complex genes is involved in OC pathogenesis. The genes may serve as potential biomarkers for OC diagnosis and prognosis.