The present study evaluated the effects of natural and artificial knitted fabrics functionalized with Cordia curassavica (Jacq.) Roem. & Schult., Boraginaceae, in the skin wound healing process induced in mice. C57BL/6 male mouse wounds were treated as follows: polylactic acid fiber; polylactic acid fiber + C. curassavica oil; cotton; and cotton + C. curassavica oil. The wound closure kinetics, histological analysis, and fibronectin mRNA expression were evaluated after 3, 5, 7, and 14 days of treatment. The wound closure kinetics area under the curve analysis evidenced the polylactic acid fiber + oil treatment accelerating the wound closure as compared to other groups (cotton: 541.7 ± 37.73; cotton + oil: 720.4 ± 27.88; polylactic acid fiber: 536.8 ± 33.89; polylactic acid fiber + oil: 945.7 ± 33.52). The skin wounds treated with functionalized fabrics showed a proper wound healing resolution, normal granulation tissue architecture and cell infiltrate, a lower number of mastocytes, a higher collagen deposition, and increased fibronectin mRNA expression levels. In summary, we report for the first time that the polylactic acid fiber accelerates the wound healing process, an effect potentialized by the C. curassavica volatile oil. These findings point to the polylactic acid fiber + oil as an important alternative therapeutic approach to improve wound healing treatment.Graphical abstract: