Abstract: We describe for the first time the nest and nestling of the Atlantic Forest endemic ochre-rumped antbird (Drymophila ochropyga) from the municipality of Vargem Alta, Espírito Santo state, southeast Brazil. In November 2020, we found a nest that was a high cup supported by vegetation at the sides, with the presence of a dome. It consisted mainly of dead leaves of bamboo and other plants, lined with thin and flexible fibers. The nest seems to differ from those of other species in the genus Drymophila in terms of shape with the presence of a dome and the lack of green moss. Nest dimensions were as follows: external height 147 mm; external diameter 119 × 97 mm; internal diameter 68 × 57 mm; and height above the ground 30 cm. The two nestlings were a few days old and had pink skin, dark plumage in a few parts of the body, and a yellow bill; their eyes were still closed. The male and female were seen feeding and brooding the nestlings, as reported for other species of Thamnophilidae. Other aspects related to the reproductive biology of the species, such as egg size, incubation period, and development of the nestlings, could not be evaluated and require future studies to achieve a better understanding of the breeding biology of this species.