INS-1 췌장 β 세포에서 Allomyrina dichotoma 혈림프 처리에 의한 early growth response protein 1 (EGR1) 유전자 발현을 조사되었다. 이 연구에서 새로운 발견은 EGR1 유전자 발현을 A. dichotoma 혈림프의 용량 및 시간 의존적으로 상향 조절하는것과 혈림프와 병행한 저체온효과 또는 소포체(endoplasmic reticulum, ER) 스트레스에 의해서도 유전자 발현이 상승하였다. A. dichotoma 혈림프가 EGR1의 유전자발현을 상승 조절을 할 수 있기 때문에, EGR1 관련 질병 치료 및 예방의 실마리를 제공 할 수 있을 가능성을 시사한다.
We have investigated the expression of early growth response protein 1 (EGR1) in INS-1 pancreatic β-cells treated with Allomyrina dichotoma hemolymph. The Korean rhinoceros beetle, A. dichotoma (Coleoptera: Scarabaeidae), is important in the insect industry for medical applications. We have already established a method for purification of A. dichotoma hemolymph that can be used in many experiments. EGR1 is reported as a multifunctional transcription factor that is implicated in virus infections. EGR1 has therefore been revealed as a major mediator and regulator in the physiological and pathological conditions of several cell and tissue types. New findings in this study are that A. dichotoma hemolymph, which promotes a dose- and time-dependent upregulation of EGR1 gene expression, shows an enhancement of this gene expression when combined with hypothermia or endoplasmic reticulum (ER) stress. These results suggest that A. dichotoma hemolymph may provide clues to EGR1-associated disease therapies involving gene regulation of EGR1.