Fluorescence is generated when a substance absorbs light energy at a short wavelength and then emits light energy at a longer wavelength. This unique characteristic evokes a “fluorescent feeling,” which is different from the perception that is detected when objects reflect light. In our previous study, when an image that was colorimetrically equivalent to a real fluorescent object was reproduced on a monitor, the fluorescent feeling evoked by the reproduced image was reduced. This suggested that the differences in spectral distribution between a reproduced image and a real fluorescent object can affect the fluorescent feeling. In recent years, the role of intrinsically photosensitive retinal ganglion cells (ipRGCs) in the detection of brightness has been reported. In this study, we investigate the contribution of ipRGCs to the fluorescent feeling. In our experiment, ipRGC stimulation was tested and compared to the light flux stimulus. We investigated both central vision and peripheral vision. We found that as ipRCG excitation increased, the participants perceived a larger fluorescent feeling. In addition, a large fluorescent feeling was perceived in the peripheral vision, where ipRGCs play a prominent role in image detection. These results indicate that ipRGCs are important for the development of the fluorescent feeling.