Sarcopenic obesity is a new class of obesity in older adults with low skeletal muscle mass coupled with high levels of adiposity. Natural product "C" used in this experiment contains a large amount of flavon luteolin and apigenin, and its efficacy on sarcopenic obesity is unknown. Therefore, to evaluate the efficacy of natural product C in sarcopenic obesity, C57BL/6J mice were fed a normal diet, high-fat (HFD, 45 kcal% fat), or HFD+0.1%(w/w) C diet for 20 weeks. The C group showed a significant effect on improving plasma lipid concentration such as plasma FFA, TG and Cholesterol, as well as improvement of adiposity and NAFLD compared to HFD group. Dietary C increased muscle weight and decreased muscular lipids levels, which may be linked to observed increases in hind leg thickness and grip strength. The results of the muscular morphological analysis showed supplement of C increased the binding force of muscle cells and improved fibrosis. Moreover, the C increased the protein expressions involved in protein synthesis (Akt, PI3K, mTOR, PGyC1α, MyD88 and Traf6) compared to the HFD group. Overall, these findings suggest that dietary C may be beneficial for improving sarcopenic obesity.