Objective: The purpose of this study is to evaluate the braking system, which affects neck of occupants directly and vehicle kinematics refers to low speed rear-end collision. Background: Since whiplash injury is the most significant injury caused by low speed rear-end collision, we particularly examined the incidence of injury in the neck when the collision happens. Although many research have previously analyzed degree of injury occurred in various collision, which were either presence or absence of seat belt, seatback angle etc., research about the influence of braking system in collision is limited. Therefore, we focused on the influence of braking system to drivers, particularly their neck, when low speed rear-end collision happens. Method: Experiments were conducted using a sled which was simulated the low speed rear-end collision. Eleven male volunteers (average age: 25.0±1.7years; height: 173.3±2.5cm; weight: 70.1±2.9kg) participated in the experiment. Two conditions were given: 1) unaware occupant without braking, 2) unaware occupant with braking. The 3D motion captured data were collected from the results of dynamic sled tests and analyzed by calculating effective impact speed and two criteria of neck injury which include neck injury criteria (NIC), shear and tensile force on the occipital condyle. Results: The average peak effective impact speed increased in collision without the braking system as compared with the braking system (P<0.005). However, the NIC peaks showed no significant differences between with and without the braking system. The shear and tensile force on the occipital condyle increased during collision with the braking system as compared without the braking system. Conclusion: When low speed rear-end collision happened, both shear force and tensile force were measured significantly higher when a braking system was activated. Also effective impact speed, which relates to the incidence of injury, tended to be higher when a braking system was activated. These results reveal that both the braking system and vehicle kinematics are considerable factors affecting neck injury of occupants when low speed rear-end collision happens.