This study composed exercise group and control group each with 10 highschool girls living in B city, conducted 12-week exercise combining aerobic and muscle exercise to examine its influence on ACTH and cortisol which is known as health –related fitness and stress index, and physiological factor. The study conclusions are as below. As for the body composition, exercise group showed significant decrease in %BF and BMI, while LBM showed significant increase. The control group did not show significant difference. The two groups did not show significant difference. As for the fitness, the exercise group showed significant increase in muscular endurance and cardiovascular endurance and control group showed no significant difference. Two groups showed significant difference in cardiovascular endurance. As for the muscular endurance and flexibility, there was no significant difference within group or between two groups. ACTH did not show significant difference within group or between two groups, Cortisol showed significant decrease in exercise group, but did not show significant difference in control group or between two groups. Therefore, the study concluded that the continuous combined exercise is beneficial to physical health by bringing positive effect on health-related fitness and stress relaxation.